{"id":5520,"date":"2018-02-23T11:18:02","date_gmt":"2018-02-23T14:18:02","guid":{"rendered":"http:\/\/en-mol.icb.usp.br\/?page_id=5520"},"modified":"2026-03-23T11:38:46","modified_gmt":"2026-03-23T14:38:46","slug":"1-27-basic-concepts","status":"publish","type":"page","link":"https:\/\/mol-en.icb.usp.br\/index.php\/1-27-basic-concepts\/","title":{"rendered":"1-27 Basic concepts"},"content":{"rendered":"<div class=\"su-row\">\n<div class=\"su-column su-column-size-1-1\"><div class=\"su-column-inner su-u-clearfix su-u-trim\">\n<strong>PREPARATION OF HARD TISSUES &#8211; BONE AND TEETH &#8211; 1 <\/strong><\/div><\/div>\n<\/div>\n<div class=\"su-row\">\n<div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\">\nThe preparation of histological sections of hard tissues is a challenge that must be solved by specific methodologies.<br \/>\nBone and teeth are rigid tissues due to the presence of a large amount of mineral salts in their extracellular spaces, mainly crystals of calcium phosphate. They are also called <strong>mineralizated<\/strong>  or <strong>calcified<\/strong> tissues.<br \/>\nAs they are very hard, regular microtomes are not suitable for sectioning them. However, special microtomes can do so. The following page describes some methods for obtaining histological sections after  dissolving and getting rid of the calcium salts.<\/p>\n<p><a href=https:\/\/mol-en.icb.usp.br\/index.php\/1-28-basic-concepts\/\">NEXT PAGE<\/a><\/p>\n<p><a href=\"https:\/\/mol-en.icb.usp.br\/index.php\/1-26-basic-concepts\/\">PREVIOUS PAGE\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/mol-en.icb.usp.br\/index.php\/1-0-basic-concepts\/\">MENU OF THIS CHAPTER<\/a><\/p>\n<\/div><\/div>\n<div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\">\n<\/div><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-5520","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/pages\/5520","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/comments?post=5520"}],"version-history":[{"count":22,"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/pages\/5520\/revisions"}],"predecessor-version":[{"id":13194,"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/pages\/5520\/revisions\/13194"}],"wp:attachment":[{"href":"https:\/\/mol-en.icb.usp.br\/index.php\/wp-json\/wp\/v2\/media?parent=5520"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}